Composite

Part:BBa_K3081009:Design

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)


pBAD-dCas9-J23119-R1+(18bp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

no


Source

E.coli

References

Qi, L. S. , Larson, M. H. , Gilbert, L. A. , Doudna, J. A. , & Lim, W. A. . (2013). Repurposing crispr as an rna-guided platform for sequence-specific control of gene expression. Cell, 152(5), 1173-1183.